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hepg2 human hepatocellular carcinoma cell lines  (ATCC)


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    ATCC hepg2 human hepatocellular carcinoma cell lines
    Tgy‐N reduces cellular senescence markers in Nutlin‐3a‐induced senescent AML12 and <t>HepG2</t> cells. (A) SA‐β‐gal staining of AML12 cells. Scale bar = 100 μm. (B) Quantitative analysis of SA‐β‐gal‐positive AML12 cells. (C) Representative Western blots of p16, p21, and GAPDH in AML12 cells. (D) Quantitative analysis of p16/GAPDH ratio in AML12 cells. (E) Quantitative analysis of p21/GAPDH ratio in AML12 cells. (F) SA‐β‐gal staining of HepG2 cells. Scale bar = 100 μm. (G) Quantitative analysis of SA‐β‐gal‐positive HepG2 cells. (H) Representative Western blots of p16, p21, and GAPDH in HepG2 cells. (I) Quantitative analysis of p16/GAPDH ratio in HepG2 cells. (J) Quantitative analysis of p21/GAPDH ratio in HepG2 cells. Data are expressed as mean ± SEM. Statistical assessments were performed using one‐way ANOVA followed by post hoc Duncan's test. Different letters above the bars denote statistically significant differences between groups at the p < 0.05 level.
    Hepg2 Human Hepatocellular Carcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 30047 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepatocellular+carcinoma+cells/Hep+G2/pmc13621814-144-5-14
    Average 99 stars, based on 30047 article reviews
    hepg2 human hepatocellular carcinoma cell lines - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Protective Effects of Nongxiang‐Type Tieguanyin on the Liver of D‐Galactose‐Induced Aging C57BL /6 Mice via the NF ‐ κB Signaling Pathway"

    Article Title: Protective Effects of Nongxiang‐Type Tieguanyin on the Liver of D‐Galactose‐Induced Aging C57BL /6 Mice via the NF ‐ κB Signaling Pathway

    Journal: Food Science & Nutrition

    doi: 10.1002/fsn3.72396

    Tgy‐N reduces cellular senescence markers in Nutlin‐3a‐induced senescent AML12 and HepG2 cells. (A) SA‐β‐gal staining of AML12 cells. Scale bar = 100 μm. (B) Quantitative analysis of SA‐β‐gal‐positive AML12 cells. (C) Representative Western blots of p16, p21, and GAPDH in AML12 cells. (D) Quantitative analysis of p16/GAPDH ratio in AML12 cells. (E) Quantitative analysis of p21/GAPDH ratio in AML12 cells. (F) SA‐β‐gal staining of HepG2 cells. Scale bar = 100 μm. (G) Quantitative analysis of SA‐β‐gal‐positive HepG2 cells. (H) Representative Western blots of p16, p21, and GAPDH in HepG2 cells. (I) Quantitative analysis of p16/GAPDH ratio in HepG2 cells. (J) Quantitative analysis of p21/GAPDH ratio in HepG2 cells. Data are expressed as mean ± SEM. Statistical assessments were performed using one‐way ANOVA followed by post hoc Duncan's test. Different letters above the bars denote statistically significant differences between groups at the p < 0.05 level.
    Figure Legend Snippet: Tgy‐N reduces cellular senescence markers in Nutlin‐3a‐induced senescent AML12 and HepG2 cells. (A) SA‐β‐gal staining of AML12 cells. Scale bar = 100 μm. (B) Quantitative analysis of SA‐β‐gal‐positive AML12 cells. (C) Representative Western blots of p16, p21, and GAPDH in AML12 cells. (D) Quantitative analysis of p16/GAPDH ratio in AML12 cells. (E) Quantitative analysis of p21/GAPDH ratio in AML12 cells. (F) SA‐β‐gal staining of HepG2 cells. Scale bar = 100 μm. (G) Quantitative analysis of SA‐β‐gal‐positive HepG2 cells. (H) Representative Western blots of p16, p21, and GAPDH in HepG2 cells. (I) Quantitative analysis of p16/GAPDH ratio in HepG2 cells. (J) Quantitative analysis of p21/GAPDH ratio in HepG2 cells. Data are expressed as mean ± SEM. Statistical assessments were performed using one‐way ANOVA followed by post hoc Duncan's test. Different letters above the bars denote statistically significant differences between groups at the p < 0.05 level.

    Techniques Used: Staining, Analysis, Western Blot

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    Cell Culture:

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described., Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously. ..

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described.12,21 Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously.12 Briefly, exponentially growing cells (1 × 104 in 180 μL) were seeded in 96-well microplates and allowed to adhere overnight. ..

    MTT Assay:

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described., Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously. ..

    Article Title: Astragalus membranaceus polysaccharide (APs) and Eugenol: Multi-target Anti-inflammatory, Antioxidant, Antimicrobial, and anticancer effects validated by in Silico studies
    Article Snippet: .. The cytotoxic effects of APS, eugenol, and their combination (APS–eugenol) on human hepatocellular carcinoma cells (HepG2, ATCC HB-8065) were assessed using the MTT assay . ..

    Article Title: Structure-Based Virtual Screening of New Skp1 Inhibitor Chemotypes Targeting F-Box Binding Interface
    Article Snippet: The resultant FP values were analyzed and plotted as bar graphs using GraphPad Prism (v6.0; San Diego, CA). .. The lung cancer cells (A549), hepatocellular carcinoma cells (HepG2), colorectal cancer cells (HCT116), and breast cancer cells (MCF-7) were originally obtained from ATCC and cultured under standard conditions as previously described.12,21 Cell viability following compound(s) treatment was evaluated using the MTT assay, as reported previously.12 Briefly, exponentially growing cells (1 × 104 in 180 μL) were seeded in 96-well microplates and allowed to adhere overnight. ..

    Article Title: Resveratrol-loaded chitosan nanoparticles and adipose-derived stem cell-conditioned medium for liver protection against ischemia-reperfusion injury.
    Article Snippet: .. Cell viability was determined using the MTT assay on human hepatocellular carcinoma cells (HepG2; ATCC). ..

    In Vitro:

    Article Title: Targeting hypertension through natural phenolics: The multifaceted role of cinnamic acid and its derivatives
    Article Snippet: In vitro - primary neonatal rat cardiomyocytes and H9c2 cells , 4 μM – 500 μM , ↓Nppb expression ↓Mitochondrial SOD ↓ STAT3 and ERK1/2 , (Cui et al. 2025). .. Cinnamic acid derivatives , In vitro - Human hepatocellular carcinoma cells (HepG2, ATCC HB-8065) and Rat cardiomyocytes (H9c2, ATCC CRL-1446) , Biologically active derivatives of CA (cinnamic acid): (R, S)-(E)-N-(1-hydroxy-3-methylbutan-2-yl) cinnamamide), (E)-3-(4-chlorophenyl)-N-(1-hydroxy-2-methylpropan-2-yl) acrylamide ) and (E)-1-(4-hydroxypiperidin-1-yl)-3-phenylprop-2-en-1-one) Concentration of 25 μM , ↓ ROSs , (Koczurkiewicz-Adamczyk et al., 2021). .. , In vivo - Male Wistar rats and spontaneously hypertensive rats (SHR) , Lyophilized gel reconstituted in serum Lycopersicum esculentum L ( Chlorogenic acid most abundant in all samples, ranging from 29.5 (Week 4) to 90.7 mg/kg body weight (Week 2) in gel and from 26.1 (Week 4) to 92.3 mg/kg body weight (Week 2) in serum and Caffeic acid 8.11 (Week 4) to 9.85 mg/kg body weight (Week 2) in gel and from 3.82 (Week 4) to 3.36 mg/kg body weight (Week 2) in serum) 400 mg of lyophilized gel in 12 mL of saline (orally for 4 weeks) , ↓ TNFα , (Marcolongo et al., 2020).

    Concentration Assay:

    Article Title: Targeting hypertension through natural phenolics: The multifaceted role of cinnamic acid and its derivatives
    Article Snippet: In vitro - primary neonatal rat cardiomyocytes and H9c2 cells , 4 μM – 500 μM , ↓Nppb expression ↓Mitochondrial SOD ↓ STAT3 and ERK1/2 , (Cui et al. 2025). .. Cinnamic acid derivatives , In vitro - Human hepatocellular carcinoma cells (HepG2, ATCC HB-8065) and Rat cardiomyocytes (H9c2, ATCC CRL-1446) , Biologically active derivatives of CA (cinnamic acid): (R, S)-(E)-N-(1-hydroxy-3-methylbutan-2-yl) cinnamamide), (E)-3-(4-chlorophenyl)-N-(1-hydroxy-2-methylpropan-2-yl) acrylamide ) and (E)-1-(4-hydroxypiperidin-1-yl)-3-phenylprop-2-en-1-one) Concentration of 25 μM , ↓ ROSs , (Koczurkiewicz-Adamczyk et al., 2021). .. , In vivo - Male Wistar rats and spontaneously hypertensive rats (SHR) , Lyophilized gel reconstituted in serum Lycopersicum esculentum L ( Chlorogenic acid most abundant in all samples, ranging from 29.5 (Week 4) to 90.7 mg/kg body weight (Week 2) in gel and from 26.1 (Week 4) to 92.3 mg/kg body weight (Week 2) in serum and Caffeic acid 8.11 (Week 4) to 9.85 mg/kg body weight (Week 2) in gel and from 3.82 (Week 4) to 3.36 mg/kg body weight (Week 2) in serum) 400 mg of lyophilized gel in 12 mL of saline (orally for 4 weeks) , ↓ TNFα , (Marcolongo et al., 2020).



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    Image Search Results


    Tgy‐N reduces cellular senescence markers in Nutlin‐3a‐induced senescent AML12 and HepG2 cells. (A) SA‐β‐gal staining of AML12 cells. Scale bar = 100 μm. (B) Quantitative analysis of SA‐β‐gal‐positive AML12 cells. (C) Representative Western blots of p16, p21, and GAPDH in AML12 cells. (D) Quantitative analysis of p16/GAPDH ratio in AML12 cells. (E) Quantitative analysis of p21/GAPDH ratio in AML12 cells. (F) SA‐β‐gal staining of HepG2 cells. Scale bar = 100 μm. (G) Quantitative analysis of SA‐β‐gal‐positive HepG2 cells. (H) Representative Western blots of p16, p21, and GAPDH in HepG2 cells. (I) Quantitative analysis of p16/GAPDH ratio in HepG2 cells. (J) Quantitative analysis of p21/GAPDH ratio in HepG2 cells. Data are expressed as mean ± SEM. Statistical assessments were performed using one‐way ANOVA followed by post hoc Duncan's test. Different letters above the bars denote statistically significant differences between groups at the p < 0.05 level.

    Journal: Food Science & Nutrition

    Article Title: Protective Effects of Nongxiang‐Type Tieguanyin on the Liver of D‐Galactose‐Induced Aging C57BL /6 Mice via the NF ‐ κB Signaling Pathway

    doi: 10.1002/fsn3.72396

    Figure Lengend Snippet: Tgy‐N reduces cellular senescence markers in Nutlin‐3a‐induced senescent AML12 and HepG2 cells. (A) SA‐β‐gal staining of AML12 cells. Scale bar = 100 μm. (B) Quantitative analysis of SA‐β‐gal‐positive AML12 cells. (C) Representative Western blots of p16, p21, and GAPDH in AML12 cells. (D) Quantitative analysis of p16/GAPDH ratio in AML12 cells. (E) Quantitative analysis of p21/GAPDH ratio in AML12 cells. (F) SA‐β‐gal staining of HepG2 cells. Scale bar = 100 μm. (G) Quantitative analysis of SA‐β‐gal‐positive HepG2 cells. (H) Representative Western blots of p16, p21, and GAPDH in HepG2 cells. (I) Quantitative analysis of p16/GAPDH ratio in HepG2 cells. (J) Quantitative analysis of p21/GAPDH ratio in HepG2 cells. Data are expressed as mean ± SEM. Statistical assessments were performed using one‐way ANOVA followed by post hoc Duncan's test. Different letters above the bars denote statistically significant differences between groups at the p < 0.05 level.

    Article Snippet: AML12 (mouse normal hepatocytes) and HepG2 (human hepatocellular carcinoma) cell lines were obtained from ATCC.

    Techniques: Staining, Analysis, Western Blot